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Mesenchymal Stromal Cells Promote Neurodifferentiation via Inducing Signaling Pathways Related to Hippo-YAP in Shh-Mediated Mouse Neural Progenitor Cells.
Mesenchymal stromal cells (MSCs) are a type of multipotent progenitor cell. They can be separated from tissue such as bone marrow, adipose tissue, and umbilical cord and they have the ability to differentiate into various specialized cells, and thus have been widely used in various fields. In addition to self-renewal, the function of MSCs is immunomodulation. However, the role of immunomodulation in MSC-mediated neurological tissue repair is not yet fully understood. Here, we investigated the role of MSCs in neurogenic differentiation of mouse neural progenitor cells (mNPCs). We found that MSCs secreted a large amount of Shh. MSCs exhibited higher levels of neural progenitor markers and higher Shh secretion than mNPCs. MSCs induced neurogenic differentiation and enhanced the neural progenitor markers Nestin and Sox2 in mNPCs. Reduction of Shh in MSCs abrogated neurogenic differentiation and inhibited Sox2 expression in mNPCs. In addition, the inhibition of Shh-related signaling by siRNA interference in MSCs decreased the levels of phospho-YAP and LATS1. However, the inhibition of Shh activity in mNPCs was not enough to induce differentiation and expression of Sox2. We also found that LATS1 knockdown in mNPCs increased the expression of Sox2 and promoted neurogenic differentiation and neurite outgrowth. Our findings indicate that MSCs promote neural progenitor cell differentiation and have neurogenic differentiation potential via Shh-mediated YAP signaling. very beginning just like f…

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yamaha cs80 profesyonel org programi indir
yamaha cs80 profesyonel org programi indir
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yamaha cs80 profesyonel org programi indir
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